Size exclusion chromatography (SEC)
This page contains a collection of information how to reference analytical data. We provide this page to describe the preferred formatting of analytical evaluations in the Chemotion repository. The given preferences follow standards of journals/publishers and other stakeholders wherever we could find them. If you have suggestions and comments: please let us know or provide your ideas via the issue tracking option. https://github.com/ComPlat/chemotion_saurus/issues Updated: 21.11.2024
Inline notation for size exclusion chromatography (SEC)
The inline notation gives a short summary of the metadata and a list of the results of the SEC measurement. To be reported are the number average molecular weight Mn in Da, the weight average molecular weight Mw in Da, and the dispersity Đ, which is defined as Mw/Mn. If applicable, the viscosity molecular weight Mv in Da or the size average molecular weight Mz in Da can be reported. The used solvent, the standard used in the calibration, and the detector should be defined. The temperature should be reported if deviating from room temperature. Further, the calibration range should be defined.
Examples for a suitable formatting:
(1) Standard example:
SEC (THF, PMMA, RID, 102–10000 Da): Mn = 20.000 Da, Mw = 25.000 Da, Đ = 1.21.
(2) Example of SEC measurement at elevated temperature:
SEC (TCB, PMMA, RID, 120 °C): Mn = 20.000 Da, Mw = 25.000 g/mol, Đ = 1.21.
Explanation:
solvent = mobile phase of the SEC instrument. In example (1), the solvent is THF
standard = polymer standard used for the calibration of the instrument and analyzing the measured sample. In example (1), the standard is PMMA
Mn = number average molecular weight
Mw = weight average molecular weight
Mv = viscosity average molecular weight
D = dispersity of the molecular weight; D = Mw/Mn
Typical conditions:
THF = solvent used as mobile phase (other possible solvents include DMAc, water, DMSO, HFIP, CHCl3, TCB)
PMMA = standard used for the calibration (other common examples: PS, dextrane)
RID = detector used to determine the signals (other common examples: UV, RALS/LALS or MALS, viscosimeter, IR)
102–10000 Da = calibration range for molecular weight determination
T in °C: temperature if deviating from room temperature